stutzeri strains, but not affiliated with one of the known genomovars (Fig. Tetrathionate is an unstable compound (69) and thus would not be expected to persist in soils. All of the isolates were Gram-negative, oxidase- and catalase-positive, curved, motile rods with polar flagella when grown anaerobically in liquid culture. 1, 28359 Bremen, Germany, Kluyver Laboratory of Biotechnology, TU Delft, Julianalaan 67, 2628 BC Delft, The Netherlands, Metabolism of thiosulfate and tetrathionate by heterotrophic bacteria, Dissimilatory reduction of inorganic sulfur by facultatively anaerobic marine bacteria, Bacterial oxidation of thiosulfate at the Black Sea, Oxidation of inorganic sulfur species by heterotetrophic microorganisms, Isolation and characterization of alkaliphilic heterotrophic bacteria oxidizing sulfur compounds to tetrathionate, Reduction of tetrathionate and thiosulfate and oxidation of sulfide in, Oxidation of thiosulfate to tetrathionate by marine pseumonad strain ChG 7-3: influence on growth and properties of oxidizing system, Sulfide and thiosulfate oxidizing bacteria in anoxic marine basins, Chemolithotrophic sulfur oxidizing bacteria from Galapagos rift hydrothermal vents, The spectrophotometric determination of nitrate and nitrate in natural waters with particular reference to seawater, Rapid micromethod for determination of nitrate in presence of nitrite for biochemical studies, Cyanolysis and spectrophotomentric estimation of trithionate in mixture with thiosulfate and tetrathionate, A procedure for isolation of DNA from microorganisms, The quantitative measurements of DNA-DNA hybridization from renaturation rates, Labelling of DNA to high specific activity in vivo by nick translation with DNA polymerase I, Cold Spring Harbor Laboratory Press, Cold Spring Harbor. 0000001815 00000 n Strain TG 3, from the Pacific Ocean, was sufficiently different from the Black Sea isolates to be considered a different species by current standards for DNA-DNA-hybridization data [29]. E. It is possible that the cytochrome cd1 in such strains was simply below the detection limit of the method. Most of the hybridization and G+C mol% measurements were done using the thermal denaturation technique [21]. Although tetrathionate was also the sole product of thiosulfate oxidation in aerobic cultures, the extent of thiosulfate oxidation was significantly higher than in the absence of oxygen . G. M.J. (, Ursing 57 33 /Root 58 0 R Search for other works by this author on: Max-Planck-Institut für Marine Mikrobiologie, Celsiusstr. Can you explain the synthesis and reaction of a Grignard Reagent. Join Yahoo Answers and get 100 points today. It is to be expected, as techniques are refined, that other strains will be classified, upon closer examination, as P. stutzeri. Search for more papers by this author. The experiments with washed cells aimed to eliminate any potential influence of organic electron donors, and to clarify the roles of the different stable nitrogen oxides in anaerobic thiosulfate oxidation. DNA was isolated and purified according to the standard protocol described by Marmur et al. Anaerobiosis was achieved by flushing the medium with sterile argon for 10–15 min. Nitrite was assayed with N-(1-naphthyl) ethylene diamine dihydrochloride [16], and nitrate by the micro-salicylate method [17] after removal of nitrite by addition of solid sulfaminic acid. B.L. Correspondence to The hydrothermal vent isolate NF-13 has been described as able to oxidize thiosulfate to polythionate under aerobic conditions, and to produce gas when incubated anaerobically in sea water medium with thiosulfate and nitrate [14]. Biden family breaks decades-long tradition this year, Pat Sajak apologizes for outburst on 'Wheel of Fortune', Manufacturing error clouds vaccine study results, Nail salons, a lifeline for immigrants, begin shuttering, Seymour, 69, clarifies remark on being able to play 25, Sick mink appear to rise from the dead in Denmark, Walmart's massive Black Friday sale just went live, Baker's backer: NFL legend still believes in young CB, Americans 'tired of COVID' have experts worried, Top Trump official issues stark COVID-19 warning. 0000098439 00000 n F. The difference cytochrome spectra were recorded with a Pye-Unicam 1800 (UK) spectrophotometer. It is therefore not surprising that denitrifying thiosulfate oxidation was much less active than in the presence of oxygen. The data in the tables represent averages of the results. The morphology of the cells within the R- and S-type colonies was sufficiently different to suggest two different species. DNA-DNA hybridization was performed by two independent procedures. The numbers 1–8 indicate genomovars [32,33]. Autotrophic growth was tested using the mineral medium without organic additions. C. R.A. Stouthamer At least two sets of measurements were made per steady state, with an interval of at least 1 volume change between measurements. Although tetrathionate was also the sole product of thiosulfate oxidation in aerobic cultures, the extent of thiosulfate oxidation was significantly higher than in the absence of oxygen (Table 2). In batch cultures with acetate as carbon and energy source, most active anaerobic thiosulfate oxidation occurred with N2O as electron acceptor. D.Yu. Y. J. 0000063123 00000 n H.G. The absorption maxima typical of this nitrite reductase (γ 460–470 nm; α 610–615 and 660–670 nm) were not observed in spectra made on similar protein samples from the group 2 isolates. Subscription will auto renew annually. L.F. J Biol Chem 193:265–275, PubMed  Jannasch PCR products were sequenced directly using the non-radioactive Taq Dyedeoxy Terminator Cycle Sequencing Kit and Applied Biosystems 373 sequencer (Applied Biosystems, Foster City, CA). E.R.B. 0000026134 00000 n Chemolithoautotrophic oxidation of thiosulfate, tetrathionate and thiocyanate by a novel rhizobacterium belonging to the genus Paracoccus. /Size 90 Thiosulfate, being a by-productofmammaliancysteine catabolism, is also pres-ent in urine (136). The pH of the medium was adjusted to 7.8 with NaHCO3. 58 0 obj /T 266306 However, in the presence of chalcopyrite which contains ferric iron, thiosulfate is rapidly and quantitatively oxidized to tetrathionate which then undergoes slow oxidation to sulfate. McCaughey E.B. /L 267490 (, Kelly P. The results presented are averages of at least five sets of data, with a variation of 5–10%.


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